Standalone applications and/or applets?
  Do you want to be able to run Python "applets" inside a web browser?

Should there be a security model that limits access to local and remote
resources so that a PythonCard app could be marked "safe"? It should be
possible to have safe apps and full apps. This is related to the issue above
of running inside a browser.

Preference for self-contained apps where the data, GUI, and any required
files are part of one package (not the same as a Python package) versus many
separate files that may be wrapped by a single directory? I lean towards the
model of a single file, maybe something like a Java .jar or .war which are
just .zip files, but with a special header file. One of the existing Python
file formats may satisfy this requirement. I also like the model of
requiring a user to have a standard Python installation (or Python runtime)
rather than every PythonCard app needing to be bundled with a standard set
of modules.

Should a PythonCard app be cross-platform and if so, should it look the same
on all platforms or conform to the local look and feel?

Any preferences on existing GUI frameworks such as tk and wxPython?

Feel free to start separate threads on each of these issues.

ka